How to Read Water Level of a Pipette

How to Read Water Level of a Pipette

A pipette is known as a graduated tube which draws in liquids for the precise volume measurement and then transfers the liquids to the other containers. Many types of pipette can be used for different applications. Some of the popular types are graduated, volumetric, mechanical action, and micro-liter pipettes that used for working in medical and scientific research environment. At this time, this article is going to deliver some information on how to read water level of a pipette. So, just take a look at the information below.

First thing is to examine the pipette closely and you should note the calibrated volume of the pipette in the millimeter units. This number is etched clearly near the top. You can find that the evenly spaced numbers and marks along the length of the pipette are the calibration marks which correspond to the precise volumes. Moreover, the volume marks which correspond to the liquid level is the liquid volume that delivered when the pipette is completely drained by the gravity.

The second thing is to draw the water unto the pipette by using the suction of pipette bulb, or by mouth, to any level in the top half of calibration range. You need to stabilize the water level before you are going to proceed. It can be done with the bulb, or by tightly stopping-up the top of the pipette with a thumb or finger.

The third thing is to raise the pipette to the eye level and you should note the curved surface which forms the top of the liquid column. After that, you can directly place the meniscus in front of your best-trained eye and make note about the calibration numbers which the meniscus falls between. The numbers can represent the fractions of the calibrated volume.

The next thing is to make note about the liquid volume. And finally, you can estimate the fractional subdivision.